Activity of Natural Tularemia Foci in West Ukraine
نویسندگان
چکیده
Methods 1. Light microscopy of smears of organs and tissues of animals, bacterial suspension (Gram staining) the study of morphological and tinctorial properties of the pathogen. 2. Immunofluorescence method for detection of antibody (IFA) detection of tularemia bacterial cells using specific fluorescent immunoglobulin. 3. Biological method subcutaneous infection of laboratory animals (white mice) with material from environmental samples and bacterial suspension (for accumulation of tularemia agent in organs and tissues of laboratory animals). 4. Bacteriological method inoculation of samples of wild and laboratory animals in differential diagnostic nutrient media (for isolation of a pure culture of tularemia agent). 5. Serological method: Indirect reaction of agglutination detection of antibodies to tularemia agent in blood of humans, wild rodents (liquid tularemia antigen erythrocyte diagnostic agent). Indirect reaction of agglutination detection of tularemia agent and its antigen in suspensions of organs, swabs of substrate from nests of rodents, pellets of birds (liquid tularemia immunoglobulin erythrocyte diagnostic agent). Reaction of agglutination detection of tularemia agent and its antigen (dry tularemia diagnostic serum).
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